Welcome to the Burton laboratory!
Our laboratory seeks to understand how cells move one of the largest and most hydrophilic biological molecules across hydrophobic membrane barriers. In bacteria alone, this process is involved in the transfer of antibiotic resistance, spore formation, and proper chromosome segregation during growth. Yet, very little is known about the molecular mechanism of DNA translocation across membranes in any system, as tools to study the mechanism of DNA transporters in their biological context at the membrane have been lacking. We combine in vitro and in vivo biochemistry, microscopy, microbiology and molecular biology to study these DNA transport complexes.
- Efficient plasmid transfer via natural competence in a microbial co-culture (2023) Molecular Systems Biology
- An electric alarm clock for spores. (2022) Science
- Natural Transformation Protein ComFA Exhibits Single-Stranded DNA Translocase Activity. (2022). Journal of bacteriology
- Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights. (2021). Nature communications
- Molecular and Cell Biological Analysis of SwrB in Bacillus subtilis. (2021). Journal of bacteriology
- Pervasive prophage recombination occurs during evolution of spore-forming Bacilli. (2020). The ISME journal
- DNA-Membrane Anchor Facilitates Efficient Chromosome Translocation at a Distance in Bacillus subtilis. (2019). mBio
- Quantitative Transformation Efficiency Assay for Bacillus subtilis . (2018). Bio-protocol
- Bacterial transformation: ComFA is a DNA-dependent ATPase that forms complexes with ComFC and DprA. (2017). Molecular microbiology
- View all lab publications